کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2582798 1561700 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and characterization of the carbonic anhydrase enzyme from Black Sea trout (Salmo trutta Labrax Coruhensis) kidney and inhibition effects of some metal ions on enzyme activity
ترجمه فارسی عنوان
تخلیص و تعیین خصوصیات آنزیم کربنیک انهیدراز از ماهی قزل آلای دریای سیاه (Salmo trutta Labrax Coruhensis) کلیه و مهار اثرات برخی یونهای فلزی بر فعالیت آنزیم
کلمات کلیدی
ماهی قزل آلا سیاه؛ Salmo trutta Labrax؛ تصفیه آنزیم؛ ویژگی های آنزیم؛ کربن انیدراز؛ فلزات سنگین
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


• Carbonic anhydrase enzyme was purified from Salmo trutta Labrax Coruhensis.
• Enzyme was characterized.
• SDS-PAGE was performed for enzyme purity and subunit.
• The effect of some heavy metals was determined on CA activity.

In this study, the carbonic anhydrase (CA) enzyme was purified from Black Sea trout (Salmo trutta Labrax Coruhensis) kidney with a specific activity of 603.77 EU/mg and a yield of 35.5% using Sepharose-4B-l-tyrosine- sulphanilamide affinity column chromatography. For determining the enzyme purity and subunit molecular mass, sodiumdodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The molecular mass of subunit was found approximately 29.71 kDa. The optimum temperature, activation energy (Ea), activation enthalpy (ΔH) and Q10 values were obtained from Arrhenius plot. Km and Vmax values for p-nitrophenyl acetate of the purified enzyme were calculated from Lineweaver-Burk graphs. In addition, the inhibitory effects of different heavy metal ions (Fe2+, Pb2+, Co2+, Ag+ and Cu2+) on Black Sea trout kidney tissue CA enzyme activities were investigated by using esterase method under in vitro conditions. The heavy metal concentrations inhibiting 50% of enzyme activity (IC50) were obtained. Finally Ki values and inhibition types were calculated from Lineweaver-Burk graphs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 44, June 2016, Pages 134–139
نویسندگان
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