Evaluation of the toxic and genotoxic potential of acid mine drainage using physicochemical parameters and bioassays

Carboniferous activity generates acid mine drainage (AMD) which is capable of unleashing toxic effects on the exposed biota. The aim of this study was to evaluate the toxic and genotoxic potential of untreated-AMD and AMD treated with calcinated sediment, using physicochemical parameters and bioassays. Results revealed that untreated-AMD presented low pH values and elevated concentrations of the metals Fe, Al, Mn, Zn and Cu. High acute toxicity was observed in Artemia sp. and Daphnia magna, and sub-chronic toxicity and genotoxicity in Allium cepa L. as well as scission of plasmid DNA exposed to untreated-AMD. Treatment of AMD with calcinated sediment promoted the reduction of acidity and the removal of metals, as well as a reduction in toxic and genotoxic effects. In conclusion, the calcinated sediment can be used as an alternative AMD treatment.

► Acid mine drainage was treated with calcinated sediment.
► Physicochemical parameters and toxicity in bioindicator organisms were evaluated.
► The treatment resulted in an increase in the pH and remove metals in the drainage.
► The toxic effects of drainage on bioindicator organisms were reduced after treatment.