Prevention of γ-radiation induced cellular genotoxicity by tempol: Protection of hematopoietic system

Tempol (TPL) under in vitro conditions reduced the extent of gamma radiation induced membrane lipid peroxidation and disappearance of covalently closed circular form of plasmid pBR322. TPL protected cellular DNA from radiation-induced damage in various tissues under ex vivo and in vivo conditions as evidenced by comet assay. TPL also prevented radiation induced micronuclei formation (in peripheral blood leucocytes) and chromosomal aberrations (in bone marrow cells) in whole body irradiated mice. TPL enhanced the rate of repair of cellular DNA (blood leucocytes and bone marrow cells) damage when administered immediately after radiation exposure as revealed from the increased Cellular DNA Repair Index (CRI). The studies thus provided compelling evidence to reveal the effectiveness of TPL to protect hematopoietic system from radiation injury.

► Tempol (TPL) reduced the extent of radiation induced membrane lipid peroxidation in vitro.
► TPL reduced the extent of radiation induced DNA strand breaks in plasmid pBR322.
► TPL protected from radiation induced cellular DNA damage ex vivo and in vivo as evidenced by comet assay.
► TPL prevented radiation induced micronuclei formation and chromosomal aberrations in whole body irradiated mice.
► TPL enhanced DNA repair in radiation exposed cells in vivo as revealed by the increased Cellular DNA Repair Index (CRI).