کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2583643 | 1130697 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Decitabine, independent of apoptosis, exerts its cytotoxic effects on cell growth in melanoma cells Decitabine, independent of apoptosis, exerts its cytotoxic effects on cell growth in melanoma cells](/preview/png/2583643.png)
Decitabine is a synthesized cytosine analog that is a potent inhibitor of DNA methylation. There have been a few reports on the in vitro anti-melanoma effect of decitabine or its functional mechanisms. We investigated the anti-proliferation effect of decitabine on the cultured murine melanoma cell line K1735M2. MTT assay showed that decitabine had strong inhibition on melanoma K1735M2 in a time- and dose-dependent manner in vitro. Morphological observation showed that decitabine could induce melanoma K1735M2 cells to produce dendrite-like structures with the increase of decitabine concentration and incubation time. Decitabine could effectively induce K1735M2 cells to differentiate in vitro. Additionally, decitabine could induce a dose-dependent G2/M cell cycle arrest in K1735M2 cells. We provided experimental evidences that the anti-proliferation effect of decitabine on murine K1735M2 melanoma cells was associated predominately with G2/M cell cycle arrest and the induction of differentiation rather than apopotosis.
► Decitabine could inhibit the proliferation of melanoma K1735M2 cells in vitro.
► Decitabine could effectively induce K1735M2 cells to differentiate in vitro.
► Decitabine could induce a dose-dependent G2/M cell cycle arrest in K1735M2 cells.
► Decitabine exerts its cytotoxic effects on cell growth in K1735M2 cells independent of apoptosis.
Journal: Environmental Toxicology and Pharmacology - Volume 32, Issue 3, November 2011, Pages 423–429