کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2583948 | 1130709 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic changes in response to acute cadmium toxicity in gill tissue of Paralichthys olivaceus
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کلمات کلیدی
Paralichthys olivaceusAAS2D-PAGEPMFGill tissueCBBIEFCBPHspCoomassie Brilliant Blue - Coomassie درخشان آبیSp1 - SP1Peptide mass fingerprinting - اثر انگشت پپتیدهmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry - اسپکترومتر جرمی زمان یخ زدن لیزر جذب / یونیزاسیون ماتریسscAT - اسکاتtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیCAM - ساخت به کمک کامپیوترInductively coupled plasma mass spectrometer - طیف سنج جرمی پلاسما به صورت القاییICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییMALDI-TOF-MS - مالدی TOF-MSSpecificity protein 1 - مشخصات پروتئین 1Proteomics - پروتئومیکسHeat shock protein - پروتئین شوک حرارتcalcium-binding protein - پروتئین متصل به کلسیمCalmodulin - کالمودولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Proteomic changes in response to acute cadmium toxicity in gill tissue of Paralichthys olivaceus Proteomic changes in response to acute cadmium toxicity in gill tissue of Paralichthys olivaceus](/preview/png/2583948.png)
چکیده انگلیسی
In the present study, we developed a two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique for examining the response of the proteome from gill tissue of Paralichthys olivaceus (POGT) to acute cadmium (AC) toxicity. Approximately 700 protein spots were detected from the gill sample when applying a 600 μg protein 2D-PAGE gel in the pH range 5.0-8.0, and approximately 400 of these were identified by peptide mass fingerprinting (PMF) and database search. Compared to a control sample, significant changes were visualized in 18 protein spots exposed to seawater cadmium acute toxicity at 10.0 ppm for 24 h. Among these spots, two were up-regulated, one was down-regulated, seven showed low expression, and eight showed high expression. The collected spots were further identified by PMF and database search. Ten of the 18 proteins identified on the 2D-PAGE gel, including heat shock protein 70 and calcium-binding protein, demonstrated a synchronous response to AC, and we suggest that the variable levels and trends of these spots on the gel might be utilized as biomarker profiles to investigate cadmium contamination levels in seawater and to evaluate the degree of risk of human fatalities. The experimental results emphasize that the application of multiple biomarkers has an advantage over single biomarkers for monitoring levels of heavy metal contamination in seawater.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 27, Issue 2, March 2009, Pages 212-218
Journal: Environmental Toxicology and Pharmacology - Volume 27, Issue 2, March 2009, Pages 212-218
نویسندگان
Xue-Ping Ling, Jin-Yong Zhu, Lin Huang, He-Qing Huang,