کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2584442 1130739 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a yeast-based assay to determine the (anti)androgenic contaminants from pulp and paper mill effluents in India
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Development of a yeast-based assay to determine the (anti)androgenic contaminants from pulp and paper mill effluents in India
چکیده انگلیسی

We have constructed an efficient and reliable yeast-based detection system to evaluate the androgenic activity of endocrine disruptors from pulp and paper mill effluents (PPME). This system consists of human androgen receptor and androgen response elements driven β-galactosidase genes transformed in yeast, Saccharomyces cerevisiae. The transcriptional activation by known androgens, correlated with androgenic activities as measured by other assay systems. This assay system when applied to evaluate anti-androgenic activities, the known anti-androgens effectively inhibited reporter gene induction by testosterone. The specificity of the assay was tested by incubating the transformed cells with supraphysiological concentrations of non-androgenic steroids and none of them gave a significant response. The extracted PPME from five different mills demonstrated strong androgenic activities (about five- to eight-folds over control). These results suggest that PPME are rich in androgenic chemicals and the employed detection system could be applicable to primary screening for effectors on androgen receptor functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 24, Issue 2, September 2007, Pages 114–121
نویسندگان
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