کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2590956 1562092 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ketamine exposure in early development impairs specification of the primary germ cell layers
ترجمه فارسی عنوان
قرار گرفتن در معرض کتامین در ابتدای کار، مشخصه های لایه های سلولی اولیه را مختل می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


• We modeled in utero NMDAr antagonism with mouse embryonic stem cells.
• We examined the effect of ketamine, MK-801 and NMDA on cell fate specification.
• NMDAr antagonism interferes with the normal developmental pathways of embryogenesis.

Preclinical and clinical evidence implicates N-methyl-d-aspartate receptor (NMDAr) signaling in early embryological development. However, the role of NMDAr signaling in early development has not been well studied. Here, we use a mouse embryonic stem cell model to perform a step-wise exploration of the effects of NMDAr signaling on early cell fate specification. We found that antagonism of the NMDAr impaired specification into the neuroectodermal and mesoendodermal cell lineages, with little or no effect on specification of the extraembryonic endoderm cell lineage. Consistent with these findings, exogenous NMDA promoted neuroectodermal differentiation. Finally, NMDAr antagonism modified expression of several key targets of TGF-β superfamily signaling, suggesting a mechanism for these findings. In summary, this study shows that NMDAr antagonism interferes with the normal developmental pathways of embryogenesis, and suggests that interference is most pronounced prior to neuroectodermal and mesoendodermal cell fate specification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurotoxicology and Teratology - Volume 43, May–June 2014, Pages 59–68
نویسندگان
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