کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2591570 1131816 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Utility of Caenorhabditis elegans in high throughput neurotoxicological research
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Utility of Caenorhabditis elegans in high throughput neurotoxicological research
چکیده انگلیسی

Caenorhabditis elegans is a nematode that has been used as a valuable research tool in many facets of biological research. Researchers have used the many tools available to investigate this well-studied nematode, including a cell lineage map, sequenced genome, and complete wiring diagram of the nervous system, making in-depth investigation of the nervous system practical. These tools, along with other advantages, such as its small size, short life cycle, transparency, and ability to generate many progeny, have made C. elegans an attractive model for many studies, including those investigating toxicological paradigms and those using high throughput techniques. Researchers have investigated a number of endpoints, such as behavior and protein expression using a green fluorescent protein (GFP) marker following toxicant exposure and have explored the mechanisms of toxicity using techniques such as microarray, RNA interference (RNAi), and mutagenesis. This review discusses the benefits of using C. elegans as a model system and gives examples of the uses of C. elegans in toxicological research. High throughput techniques are discussed highlighting the advantages of using an in vivo system that has many advantageous characteristics of an in vitro system while emphasizing endpoints relating to developmental and adult neurotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurotoxicology and Teratology - Volume 32, Issue 1, January–February 2010, Pages 62–67
نویسندگان
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