کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2596022 | 1562370 | 2011 | 6 صفحه PDF | دانلود رایگان |

Studies showed that certain cytotoxicity assays were not suitable for assessing nanoparticle (NP) toxicity. We evaluated a lactate dehydrogenase (LDH) assay for assessing copper (Cu-40, 40 nm), silver (Ag-35, 35 nm; Ag-40, 40 nm), and titanium dioxide (TiO2-25, 25 nm) NPs by examining their potential to inactivate LDH and interference with β-nicotinamide adenine dinucleotide (NADH), a substrate for the assay. We also performed a dissolution assay for some of the NPs. We found that the copper NPs, because of their high dissolution rate, could interfere with the LDH assay by inactivating LDH. Ag-35 could also inactivate LDH probably because of the carbon matrix used to cage the particles during synthesis. TiO2-25 NPs were found to adsorb LDH molecules. In conclusion, NP interference with the LDH assay depends on the type of NPs and the suitability of the assay for assessing NP toxicity should be examined case by case.
► We examined if some nanoparticles (NPs) can interfere with an LDH assay.
► Copper NP and a silver NP could interfere with the assay by inactivating LDH.
► Titanium dioxide NPs were found to adsorb LDH but only at high doses.
► Thus, the validity of the assay for assessing NP toxicity depends on NP types.
Journal: Toxicology - Volume 287, Issues 1–3, 5 September 2011, Pages 99–104