کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2596616 | 1562394 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diverse chemicals including aryl hydrocarbon receptor ligands modulate transcriptional activity of the 3â²immunoglobulin heavy chain regulatory region
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کلمات کلیدی
HAHsARNTDRERT-PCRIgHIC50TCDDICZAP-1BITCLPSAHRNF-1PBSNF-κB2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسین50% inhibitory concentration - 50٪ غلظت مهاریDMSO - DMSOOct - اکتبرimmunoglobulin - ایمونوگلوبولینBenzyl isothiocyanate - بنزیل ایزوتیوسیاناتELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاIn vitro - درونکشتگاهیDimethyl sulfoxide - دیمتیل سولفواکسیدImmunoglobulin heavy chain - زنجیره سنگین ایمونوگلوبولینvariable heavy chain - زنجیره سنگین متغیرdioxin responsive element - عنصر پاسخگو دیوکسینnuclear factor-κB - فاکتور هسته ای κBAhR nuclear translocator - فرستنده هسته ای AhRPhosphate buffered saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکاریدneurofibromatosis type 1 - نورفیبروماتوز نوع یکPolycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایPAHs - هیدروکربن های آروماتیک چند حلقه ایHalogenated aromatic hydrocarbons - هیدروکربن های معطر هالوژنیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسactivator protein 1 - پروتئین فعال کننده 1aryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Diverse chemicals including aryl hydrocarbon receptor ligands modulate transcriptional activity of the 3â²immunoglobulin heavy chain regulatory region Diverse chemicals including aryl hydrocarbon receptor ligands modulate transcriptional activity of the 3â²immunoglobulin heavy chain regulatory region](/preview/png/2596616.png)
چکیده انگلیسی
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a known disruptor of B-cell differentiation and a ligand for the aryl hydrocarbon receptor (AhR), induces binding of the AhR to dioxin responsive elements (DRE) in sensitive genes. The Ig heavy chain (IgH) gene is a sensitive target of TCDD and may be transcriptionally inhibited by TCDD through inhibition of the 3â²IgH transcriptional regulatory region (3â²IgHRR). While the 3â²IgHRR contains binding sites for several transcription factors, two DRE motifs were also identified which may be responsible for TCDD-induced inhibition of 3â²IgHRR activation and may implicate the AhR as an important regulator of IgH expression. The objectives of the present study were to determine if 3â²IgHRR modulation is limited to TCDD or if structurally diverse chemicals (AhR ligands and non-AhR ligands) from environmental, industrial, dietary or pharmaceutical origin are also capable of modulating the 3â²IgHRR and to verify a correlation between effects on a stable 3â²IgHRR reporter and the endogenous IgH protein. Utilizing a CH12.LX mouse B-cell line that stably expresses a 3â²IgHRR-regulated transgene, we identified an inhibition of both 3â²IgHRR activation and IgH protein expression by the non-dioxin AhR activators indolo(3,2-b)carbazole, primaquine, carbaryl, and omeprazole which followed a rank order potency for AhR activation supporting a role of the AhR in the transcriptional regulation of the 3â²IgHRR and IgH expression. However, modulation of the 3â²IgHRR and IgH expression was not limited to AhR activators or to suppressive effects. Hydrogen peroxide and terbutaline had an activating effect and benzyl isothiocyanate was inhibitory. These chemicals are not known to influence the AhR signaling pathway but have been previously shown to modulate humoral immunity and/or transcription factors that regulate the 3â²IgHRR. Taken together these results implicate the 3â²IgHRR as a sensitive immunological target and are the first to identify altered 3â²IgHRR activation by a diverse range of chemicals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 261, Issues 1â2, 30 June 2009, Pages 9-18
Journal: Toxicology - Volume 261, Issues 1â2, 30 June 2009, Pages 9-18
نویسندگان
Rebecca A. Henseler, Eric J. Romer, Courtney E.W. Sulentic,