کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2597070 1562402 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells
چکیده انگلیسی

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography–tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The present study demonstrated the morphological effects as well as affected proteins in Cd-treated cortical cells, providing tools and insights in elucidating mechanisms how low-dose Cd distorts brain development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 253, Issues 1–3, 20 November 2008, Pages 110–116
نویسندگان
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