کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2597203 1132572 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of Kupffer cell inactivation on cycloheximide-induced hepatic injury
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Influence of Kupffer cell inactivation on cycloheximide-induced hepatic injury
چکیده انگلیسی

In our previous study, we found that cycloheximide (CHX) induces hepatocellular necrosis as well as hepatocellular apoptosis. This article evaluates the role of Kupffer cells on cycloheximide-induced hepatic injury using gadolinium chloride (GdCl3) for the inhibition of Kupffer cells. One group of rats was treated with CHX (CHX group), and another was treated with GdCl3 before being treated with the same dose of CHX (GdCl3/CHX group). The necrotic change in the GdCl3/CHX group was exacerbated under the induction of hepatocellular apoptosis by the CHX treatment. A substantial diminution of the number of ED1- or ED2-positive cells was demonstrated in the GdCl3/CHX group compared to the CHX group. In addition, the degree of decrease in ED2-positive cells was more apparent than that in ED1-positive cells. Increases in the mRNA levels of IL-10 and Stat3 were observed in the CHX group, but not in the GdCl3/CHX group. On the other hand, the hepatic mRNA levels of chemokines and adhesion molecules such as Ccl20, LOX-1, and E-selectin were significantly increased only in the GdCl3/CHX group. Thus, Kupffer cell inactivation by the GdCl3 treatment leads to a loss of the capacity to produce IL-10, supposedly resulting in the enhancement of pro-inflammatory cytokine activities such as tumor necrosis factor (TNF) signaling. These events are suggested to be a factor of the inflammatory exacerbation in the livers of the GdCl3/CHX group. In conclusion, Kupffer cells may play a role in protecting hepatic necroinflammatory changes by releasing anti-inflammatory cytokines following the hepatocellular apoptosis resulting from CHX treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 241, Issue 3, 30 November 2007, Pages 106–118
نویسندگان
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