کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2597582 1562428 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of ferulic acid on γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Influence of ferulic acid on γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes
چکیده انگلیسی

Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species (ROS) resulting in imbalance of the pro-oxidant and antioxidant activities ultimately resulting in cell death. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn, and ricebran. FA exhibit a wide range of pharmacological effects including antiageing, anti-inflammatory, anticancer, antidiabetic, antiapoptotic, and neuroprotective. The present work is aimed at evaluating the radioprotective effect of FA, on γ-radiation induced toxicity in primary cultures of isolated rat hepatocytes. Hepatocytes were isolated from the liver of rats by collagenase perfusion. The cellular changes were estimated using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS), the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH), ceruloplasmin, Vitamins A, E and C and uric acid. DNA damage was analyzed by single cell gel electrophoresis (comet assay). An increase in the severity of DNA damage was observed with increasing dose (1, 2 and 4 Gy) of γ-radiation in cultured hepatocytes. TBARS were increased significantly, whereas the levels of GSH, Vitamins C, E and A, ceruloplasmin, uric acid and antioxidant enzymes were significantly decreased in γ-irradiated groups. The maximum damage to hepatocytes was observed at 4 Gy irradiation. Pretreatment with FA (1, 5 and 10 μg/ml) significantly decrease the levels of TBARS and DNA damage. In addition, pretreatment with FA significantly increased antioxidant enzymes, GSH, Vitamins A, E and C, uric acid and ceruloplasmin levels. The maximum protection of hepatocytes was observed at 10 μg/ml of FA pretreatment. Thus, pretreatment with FA helps in protecting the hepatocytes against γ-radiation induced cellular damage and can be developed as a effective radioprotector during radiotherapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 228, Issues 2–3, 7 December 2006, Pages 249–258
نویسندگان
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