کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2636892 1563480 2014 7 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Microbiological analysis of environmental samples collected from child care facilities in North and South Carolina
ترجمه فارسی عنوان
تجزیه و تحلیل میکروبیولوژیکی نمونه های محیطی که از تاسیسات مراقبت از کودکان در کارولینای شمالی و جنوبی جمع آوری شده است
کلمات کلیدی
امکانات مراقبت از کودکان؛ مراقبت روزانه؛ نمونه برداری محیطی؛ شاخص های میکروبیولوژیک؛ پاتوژن های انتروکوک
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
چکیده انگلیسی


• Surface and care providers' hands from 40 child care facilities in North and South Carolina were subjected to microbiological analysis.
• Overall, hands had higher microbial loads than surfaces.
• Biotype I Escherichia coli was absent; pathogens Salmonella enterica, E coli O157, Campylobacter jejuni, and Shigella spp were also absent.
• Four samples showed evidence of human norovirus contamination.
• Results suggest that these facilities practiced good hygiene and sanitation.

BackgroundChildren cared for outside the home are at an increased risk of enteric disease. Microbiological analyses were performed on environmental samples collected from child care facilities in North and South Carolina.MethodsThere were 326 samples collected from 40 facilities corresponding to common surfaces (77% of samples) and the hands of care providers (23% of samples). Samples were analyzed for total aerobic plate counts (APCs), total coliforms, biotype I Escherichia coli, and pathogens Shigella spp, Salmonella enterica, E coli O157, Campylobacter jejuni, and human norovirus.ResultsMedian APCs and coliform counts for hands were 4.6 and 1.0 log10 colony-forming units (CFU) per hand, respectively. Median APCs for surfaces were 2.0 and 2.6 log10 CFU for flat and irregular surfaces, respectively. Coliforms were detected in 16% of samples, with counts ranging from 1.0 log10 to >4.3 log10 CFU, with higher counts most often observed for hand rinse samples. Biotype I E coli counts were below assay detection limits (<1 log10 CFU) for all but 1 sample. No samples were positive for any of the 4 bacterial pathogens, whereas 4 samples showed evidence of human norovirus RNA.ConclusionThe relative absence of pathogens and biotype I E coli in environmental samples suggests the child care facilities sampled in this study managed fecal contamination well.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: American Journal of Infection Control - Volume 42, Issue 10, October 2014, Pages 1049–1055
نویسندگان
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