کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2775232 | 1152317 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Growth and differentiation factor-5 (GDF-5) in the human intervertebral annulus cells and its modulation by IL-1à and TNF-α in vitro
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
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چکیده انگلیسی
Growth and differentiation factor-5 (GDF-5) is a member of the TGF-à superfamily which regulates cell division and differentiation. GDF-5 attracted high interest because of its role in skeletal development, especially in cartilaginous sites. Little is known, however, about the role of GFD-5 in disc cell biology. The present work demonstrated the immunohistologic presence of GDF-5 in human outer and inner annulus tissue. Microarray analysis of annulus cells showed significant upregulation of GDF-5 expression in herniated vs. non-herniated lumbar discs (2.14-fold change, p = 0.021). In vitro three-dimensional culture studies challenged human annulus cells with IL-1à and TNF-α, two proinflammatory cytokines known to be elevated in the human degenerating disc. Exposure resulted in significant downregulation of GDF-5 during both TNF-α exposure (5.83-fold change, p = 0.044) and IL-1à exposure (3.38-fold change, p = 0.015). In vitro findings suggest that the degenerating disc milieu, with high proinflammatory cytokine levels, may limit expression of GDF-5, resulting in limited regenerative capacity of the intact disc.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 96, Issue 2, April 2014, Pages 225-229
Journal: Experimental and Molecular Pathology - Volume 96, Issue 2, April 2014, Pages 225-229
نویسندگان
Helen E. Gruber, Gretchen L. Hoelscher, Jane A. Ingram, Synthia Bethea, Edward N. Jr.,