کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2779278 1153257 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative trait loci for bone mineral density and femoral morphology in an advanced intercross population of mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Quantitative trait loci for bone mineral density and femoral morphology in an advanced intercross population of mice
چکیده انگلیسی

Osteoporosis, characterized by low levels of bone mineral density (BMD), is a prevalent medical condition in humans. We investigated its genetic and environmental basis by searching for quantitative trait loci (QTLs) affecting six skeletal (including three BMD) traits in a G10 advanced intercross population produced from crosses of mice from the inbred strain C57BL/6J with mice from a strain selected for high voluntary wheel running. The mice in this population were fed either a high-fat or a matched control diet throughout the study, allowing us to test for QTL by diet interactions for the skeletal traits. Our genome scan uncovered a number of QTLs, the great majority of which were different from QTLs previously found for these same traits in an earlier (G4) generation of the same intercross. Further, the confidence intervals for the skeletal trait QTLs were reduced from an average of 18.5 Mb in the G4 population to an equivalent of about 9 Mb in the G10 population. We uncovered a total of 50 QTLs representing 32 separate genomic sites affecting these traits, with a distal region on chromosome 1 harboring several QTLs with large effects on the BMD traits. One QTL was located on chromosome 5 at 4.0 Mb with a confidence interval spanning from 4.0 to 4.6 Mb. Only three protein coding genes reside in this interval, and one of these, Cyp51, is an attractive candidate as others have shown that developing Cyp51 knockout embryos exhibit shortened and bowed limbs and synotosis of the femur and tibia. Several QTLs showed significant interactions with sex, although only two QTLs interacted with diet, both affecting only mice fed the high-fat diet.


► We uncovered 50 quantitative trait loci (QTLs) affecting six skeletal traits in an intercross population of mice.
► Several QTLs on distal chromosome 1 had large effects on the bone mineral density (BMD) traits.
► Cyp51 is an attractive candidate for a BMD QTL located on chromosome 5.
► Diet and sex altered the presence and/or effects of some of the skeletal QTLs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 55, Issue 1, July 2013, Pages 222–229
نویسندگان
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