کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2779983 1153288 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ribosome inactivating protein B-chain induces osteoclast differentiation from monocyte/macrophage lineage precursor cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Ribosome inactivating protein B-chain induces osteoclast differentiation from monocyte/macrophage lineage precursor cells
چکیده انگلیسی

Human osteoclast formation from mononuclear phagocyte precursors involves interactions between lectins and their receptors. A type-2 ribosome inactivating protein consists of an A chain and a B chain. The glycosylated B chain binds specifically to galactose moieties of sugar molecules. In this study we showed that the recombinant ribosome inactivating protein B-chain (rRBC) could induce osteoclast formation from human monocytes and murine RAW264.7 macrophages. Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays demonstrated that differentiation of osteoclast-like cells was induced in the presence of rRBC in a dose-dependent manner. The rRBC-induced osteoclast differentiation was independent of caspase activation and apoptosis induction activity; however, rRBC-induced osteoclastogenesis was dependent on activation of NF-κB, ERK1/2, and p38 MAP kinase. Thus, our data demonstrated that rRBC induced osteoclast differentiation through a non-apoptotic signaling pathway. In addition to triggering apoptosis, the rRBC also induced osteoclast differentiation. According to this study, a novel role is proposed for rRBC in regulating osteoclast differentiation and in osteoimmunology.

Research Highlights
► Human osteoclast formation from mononuclear phagocyte precursors involves interactions between lectins and their receptors.
► In this study we show that the recombinant ribosome inactivating protein B-chain (rRBC) can induce osteoclast formation.
► The rRBC-induced osteoclastogenesis was dependent on activation of NF-κB, ERK, and p38 MAP kinase.
► Our data demonstrate that rRBC induces osteoclast differentiation through a non-apoptotic signaling pathway.
► Our study presents a novel role for rRBC in regulating osteoclast differentiation and in osteoimmunology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 48, Issue 6, 1 June 2011, Pages 1336–1345
نویسندگان
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