کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2779983 | 1153288 | 2011 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ribosome inactivating protein B-chain induces osteoclast differentiation from monocyte/macrophage lineage precursor cells Ribosome inactivating protein B-chain induces osteoclast differentiation from monocyte/macrophage lineage precursor cells](/preview/png/2779983.png)
Human osteoclast formation from mononuclear phagocyte precursors involves interactions between lectins and their receptors. A type-2 ribosome inactivating protein consists of an A chain and a B chain. The glycosylated B chain binds specifically to galactose moieties of sugar molecules. In this study we showed that the recombinant ribosome inactivating protein B-chain (rRBC) could induce osteoclast formation from human monocytes and murine RAW264.7 macrophages. Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays demonstrated that differentiation of osteoclast-like cells was induced in the presence of rRBC in a dose-dependent manner. The rRBC-induced osteoclast differentiation was independent of caspase activation and apoptosis induction activity; however, rRBC-induced osteoclastogenesis was dependent on activation of NF-κB, ERK1/2, and p38 MAP kinase. Thus, our data demonstrated that rRBC induced osteoclast differentiation through a non-apoptotic signaling pathway. In addition to triggering apoptosis, the rRBC also induced osteoclast differentiation. According to this study, a novel role is proposed for rRBC in regulating osteoclast differentiation and in osteoimmunology.
Research Highlights
► Human osteoclast formation from mononuclear phagocyte precursors involves interactions between lectins and their receptors.
► In this study we show that the recombinant ribosome inactivating protein B-chain (rRBC) can induce osteoclast formation.
► The rRBC-induced osteoclastogenesis was dependent on activation of NF-κB, ERK, and p38 MAP kinase.
► Our data demonstrate that rRBC induces osteoclast differentiation through a non-apoptotic signaling pathway.
► Our study presents a novel role for rRBC in regulating osteoclast differentiation and in osteoimmunology.
Journal: Bone - Volume 48, Issue 6, 1 June 2011, Pages 1336–1345