کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2781989 | 1153340 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cells The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cells](/preview/png/2781989.png)
The human osteosarcoma-derived cell line, SAOS-2, exhibits many of the phenotypic characteristics of osteoblasts including the deposition of types I and V collagens in an extracellular matrix. Lesser amounts of collagen XI chains were also detected. The cell layer collagen contains hydroxylysyl pyridinoline cross-links but without the accompanying lysyl pyridinoline typical of human bone collagen. This indicates that the lysine residues at the two helical cross-linking loci are fully hydroxylated. The isoform of lysyl hydroxylase, LH1, known to be required for full hydroxylation at these sites, was shown to be highly expressed by SAOS-2 cells. Our findings provide insight on the mechanism of post-translational overmodification of lysine residues in collagen made by osteosarcoma tumors, and may be relevant for understanding a similar overmodification observed in osteoporotic bone.
Journal: Bone - Volume 40, Issue 5, May 2007, Pages 1343–1351