کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2786663 | 1568449 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of aluminium on NTPDase and 5â²-nucleotidase activities from rat synaptosomes and platelets
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
Aluminium (Al), a neurotoxic compound, has been investigated in a large number of studies both in vivo and in vitro. In this study, we investigated the effect in vivo of long-term exposure to Al on NTPDase (nucleoside triphosphate diphosphohydrolase) and 5â²-nucleotidase activities in the synaptosomes (obtained from the cerebral cortex and hippocampus) and platelets of rats. Here, we investigated a possible role of platelets as peripheral markers in rats. Rats were loaded by gavage with AlCl3 50 mg/(kg day), 5 days per week, totalizing 60 administrations. The animals were divided into four groups: (1) control (C), (2) 50 mg/kg of citrate solution (Ci), (3) 50 mg/kg of Al plus citrate (Al + Ci) solution and (4) 50 mg/kg of Al (Al). ATP hydrolysis was increased in the synaptosomes from the cerebral cortex by 42.9% for Al + Ci and 39.39% for Al, when compared to their respective control (p < 0.05). ADP hydrolysis was increased by 13.15% for both Al and Al + Ci, and AMP hydrolysis increased by 32.7% for Al and 27.25% for Al + Ci (p < 0.05). In hippocampal synaptosomes, the hydrolysis of ATP, ADP and AMP, was increased by 58.5%, 28.5% and 25.92%, respectively, for Al (p < 0.05) and 36.7%, 22.5% and 37.64% for Al + Ci, both when compared to their respective controls. ATP, ADP and AMP hydrolysis, in platelets, was increased by 172.3%, 188.52% and 92.1%, respectively in Al + Ci, and 317.9%, 342.8% and 177.9%, respectively, for Al, when compared to their respective controls (p < 0.05). Together, these results indicate that Al increases NTPDase and 5â²-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Developmental Neuroscience - Volume 25, Issue 6, October 2007, Pages 381-386
Journal: International Journal of Developmental Neuroscience - Volume 25, Issue 6, October 2007, Pages 381-386
نویسندگان
Rosilene Rodrigues Kaizer, Paula Acosta Maldonado, Rosélia Maria Spanevello, MaÃsa C. Corrêa, Jamile F. Gonçalves, Lara Vargas Becker, Vera Maria Morsch, Maria Rosa Chitolina Schetinger,