کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2792576 | 1155064 | 2016 | 14 صفحه PDF | دانلود رایگان |
• ΔΨm-based sorting segregates short-lived effector from memory T cell precursors
• Low-ΔΨm CD8+ T cells demonstrate decreased oxidative stress
• Low-ΔΨm T cells demonstrate superior antitumor activity
• Low-ΔΨm marks self-renewing hematopoietic stem cells
SummaryLong-term survival and antitumor immunity of adoptively transferred CD8+ T cells is dependent on their metabolic fitness, but approaches to isolate therapeutic T cells based on metabolic features are not well established. Here we utilized a lipophilic cationic dye tetramethylrhodamine methyl ester (TMRM) to identify and isolate metabolically robust T cells based on their mitochondrial membrane potential (ΔΨm). Comprehensive metabolomic and gene expression profiling demonstrated global features of improved metabolic fitness in low-ΔΨm-sorted CD8+ T cells. Transfer of these low-ΔΨm T cells was associated with superior long-term in vivo persistence and an enhanced capacity to eradicate established tumors compared with high-ΔΨm cells. Use of ΔΨm-based sorting to enrich for cells with superior metabolic features was observed in CD8+, CD4+ T cell subsets, and long-term hematopoietic stem cells. This metabolism-based approach to cell selection may be broadly applicable to therapies involving the transfer of HSC or lymphocytes for the treatment of viral-associated illnesses and cancer.
Graphical AbstractFigure optionsDownload high-quality image (253 K)Download as PowerPoint slide
Journal: - Volume 23, Issue 1, 12 January 2016, Pages 63–76