TRIAP1/PRELI Complexes Prevent Apoptosis by Mediating Intramitochondrial Transport of Phosphatidic Acid

• p53-regulated TRIAP1 assembles with PRELI in the mitochondrial IMS
• TRIAP1/PRELI complexes are required for CL accumulation and cell survival
• TRIAP1/PRELI complexes transfer phosphatidic acid across the IMS of mitochondria
• Excess phosphatidylglycerol ensures survival of TRIAP1- and PRELI-deficient cells

SummaryCardiolipin (CL), a mitochondria-specific glycerophospholipid, is required for diverse mitochondrial processes and orchestrates the function of various death-inducing proteins during apoptosis. Here, we identify a complex of the p53-regulated protein TRIAP1 (p53CSV) and PRELI in the mitochondrial intermembrane space (IMS), which ensures the accumulation of CL in mitochondria. TRIAP1/PRELI complexes exert lipid transfer activity in vitro and supply phosphatidic acid (PA) for CL synthesis in the inner membrane. Loss of TRIAP1 or PRELI impairs the accumulation of CL, facilitates the release of cytochrome c, and renders cells vulnerable to apoptosis upon intrinsic and extrinsic stimulation. Survival of TRIAP1- and PRELI-deficient cells is conferred by an excess of exogenously provided phosphatidylglycerol. Our results reveal a p53-dependent cell-survival pathway and highlight the importance of the CL content of mitochondrial membranes in apoptosis.

Graphical AbstractFigure optionsDownload high-quality image (228 K)Download as PowerPoint slide