کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2793682 1155170 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of Gluconeogenesis by Krüppel-like Factor 15
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Regulation of Gluconeogenesis by Krüppel-like Factor 15
چکیده انگلیسی

SummaryIn the postabsorptive state, certain tissues, including the brain, require glucose as the sole source of energy. After an overnight fast, hepatic glycogen stores are depleted, and gluconeogenesis becomes essential for preventing life-threatening hypoglycemia. Mice with a targeted deletion of KLF15, a member of the Krüppel-like family of transcription factors, display severe hypoglycemia after an overnight (18 hr) fast. We provide evidence that defective amino acid catabolism promotes the development of fasting hypoglycemia in KLF15−/− mice by limiting gluconeogenic substrate availability. KLF15−/− liver and skeletal muscle show markedly reduced mRNA expression of amino acid-degrading enzymes. Furthermore, the enzymatic activity of alanine aminotransferase (ALT), which converts the critical gluconeogenic amino acid alanine into pyruvate, is decreased (∼50%) in KLF15−/− hepatocytes. Consistent with this observation, intraperitoneal injection of pyruvate, but not alanine, rescues fasting hypoglycemia in KLF15−/− mice. We conclude that KLF15 plays an important role in the regulation of gluconeogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 5, Issue 4, 4 April 2007, Pages 305–312
نویسندگان
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