Photoperiodic control of testicular growth, histomorphology and serum testosterone levels in the male Eurasian tree sparrow: Involvement of circadian rhythm

• Circadian rhythm is involved in photoperiodic control of reproduction in tree sparrow.
• Light cycles acting as long day induce testes growth and increase testosterone level.
• Testes show different stages of spermatogenesis only in gonadostimulatory cycles.
• Histomorphometric study of testes reveal correlated variations in various structures.

Experiments were performed on the subtropical population of male Eurasian tree sparrow (Passer montanus) to examine the mediation of the circadian rhythms in photoperiodic regulation of reproductive responses. In the first experiment, photosensitive sparrows were exposed to different resonance light dark cycles viz. 6L/6D, 6L/18D, 6L/30D, 6L/42D, 6L/54D and 6L/66D along with a control group under long day length (14L/10D) for 35 days. The birds read the cycles of 6L/6D, 6L/30D and 6L/54D as long day and exhibited significant testicular growth and increased testosterone levels while the cycles of 6L/18D, 6L/42D and 6L/66D were read as short day with no testicular response. In the second experiment, groups of photosensitive birds were subjected to various intermittent light dark cycles of 2L/2D, 3L/3D, 4L/4D, 6L/6D, 8L/8D and 12L/12D with two control groups kept under 9L/15D and 14L/10D for 35 days. The birds held under the light/dark cycles of 2L/2D, 3L/3D, 4L/4D, 6L/6D and 12L/12D showed testicular growth and increased serum levels of testosterone while those exposed to 8L/8D did not. The responses were significantly higher in the birds exposed to 2L/2D, 3L/3D, 4L/4D and 6L/6D when compared to 12L/12D. Histomorphology of testes revealed different stages of spermatogenesis only under gonadostimulatory light regimes. The germinative epithelium thickness and diameter of seminiferous tubules increase while the thickness of testicular wall and area of interstitial space decrease with the increase in testicular volume. The above results indicate the involvement of an endogenous circadian rhythm in photoperiodic induction of testicular growth and functions.

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