Circadian melatonin production develops faster in birds than in mammals

The development of circadian rhythmicity of melatonin biosynthesis in the pineal gland starts during embryonic period in birds while it is delayed to the postnatal life in mammals. Daily rhythms of melatonin in isolated pinealocytes and in intact pineal glands under in vivo conditions were demonstrated during the last third of embryonic development in chick embryos, with higher levels during the dark (D) than during the light (L) phase. In addition to the LD cycle, rhythmic temperature changes with the amplitude of 4.5 °C can entrain rhythmic melatonin biosynthesis in chick embryos, with higher concentrations found during the low-temperature phase (33.0 vs 37.5 °C). Molecular clockwork starts to operate during the embryonic life in birds in line with the early development of melatonin rhythmicity. Expression of per2 and cry genes is rhythmic at least at day 16 and 18, respectively, and the circadian system operates in a mature-like manner soon after hatching. Rhythmic oscillations are detected earlier in the central oscillator (the pineal gland) than in the peripheral structures, reflecting the synchronization of individual cells which is necessary for detection of the rhythm. The early development of the circadian system in birds reflects an absence of rhythmic maternal melatonin which in mammals synchronizes physiological processes of offspring. Developmental consequences of modified development of circadian system for its stability later in development are not known and should be studied.

Research highlights
► Circadian pineal melatonin biosynthesis starts during embryonic period in birds.
► Both light:dark and temperature cycle entrain melatonin biosynthesis in chick embryos.
► Molecular clockwork starts to operate during the embryonic life in birds.
► Rhythms are detected earlier in the central oscillator than in peripheral structures.