کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2805973 1157091 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapamycin does not affect post-absorptive protein metabolism in human skeletal muscle
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Rapamycin does not affect post-absorptive protein metabolism in human skeletal muscle
چکیده انگلیسی

Administration of the mTORC1 inhibitor, rapamycin, to humans blocks the increase in skeletal muscle protein synthesis in response to resistance exercise or amino acid ingestion.ObjectiveTo determine whether rapamycin administration influences basal post-absorptive protein synthesis or breakdown in human skeletal muscle.Materials/MethodsSix young (26 ± 2 years) subjects were studied during two separate trials, in which each trial was divided into two consecutive 2 h basal periods. The trials were identical except during one trial a single oral dose (16 mg) of rapamycin was administered immediately prior to the second basal period. Muscle biopsies were obtained from the vastus lateralis at 0, 2, and 4 h to examine protein synthesis, mTORC1 signaling, and markers of autophagy (LC3B-I and LC3B-II protein) associated with each 2 h basal period.ResultsDuring the Control trial, muscle protein synthesis, whole body protein breakdown (phenylalanine Ra), mTORC1 signaling, and markers of autophagy were similar between both basal periods (p > 0.05). During the Rapamycin trial, these variables were similar to the Control trial (p > 0.05) and were unaltered by rapamycin administration (p > 0.05). Thus, post-absorptive muscle protein metabolism and mTORC1 signaling were not affected by rapamycin administration.ConclusionsShort-term rapamycin administration may only impair protein synthesis in human skeletal muscle when combined with a stimulus such as resistance exercise or increased amino acid availability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolism - Volume 62, Issue 1, January 2013, Pages 144–151
نویسندگان
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