کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815302 1159864 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reference gene selection for quantitative gene expression studies during biological invasions: A test on multiple genes and tissues in a model ascidian Ciona savignyi
ترجمه فارسی عنوان
انتخاب ژن مرجع برای مطالعات کمی بیان ژن در هنگام حمله های بیولوژیکی: آزمایش بر روی ژن ها و بافت های مختلف در یک مدل آسسییدان سیونا واجنین
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی


• For quantitative gene expression studies, reference gene selection is crucial to ensure accurate results.
• The stability of reference genes were assessed by geNorm, NormFinder and delta Ct method.
• We recommend reference gene combinations in multiple tissues under two abiotic stresses for a model ascidian Ciona savignyi in marine ecosystems.
• We carried out a case study that two target genes were normalized and analyzed using the selected reference genes.

As invasive species have successfully colonized a wide range of dramatically different local environments, they offer a good opportunity to study interactions between species and rapidly changing environments. Gene expression represents one of the primary and crucial mechanisms for rapid adaptation to local environments. Here, we aim to select reference genes for quantitative gene expression analysis based on quantitative Real-Time PCR (qRT-PCR) for a model invasive ascidian, Ciona savignyi. We analyzed the stability of ten candidate reference genes in three tissues (siphon, pharynx and intestine) under two key environmental stresses (temperature and salinity) in the marine realm based on three programs (geNorm, NormFinder and delta Ct method). Our results demonstrated only minor difference for stability rankings among the three methods. The use of different single reference gene might influence the data interpretation, while multiple reference genes could minimize possible errors. Therefore, reference gene combinations were recommended for different tissues - the optimal reference gene combination for siphon was RPS15 and RPL17 under temperature stress, and RPL17, UBQ and TubA under salinity treatment; for pharynx, TubB, TubA and RPL17 were the most stable genes under temperature stress, while TubB, TubA and UBQ were the best under salinity stress; for intestine, UBQ, RPS15 and RPL17 were the most reliable reference genes under both treatments. Our results suggest that the necessity of selection and test of reference genes for different tissues under varying environmental stresses. The results obtained here are expected to reveal mechanisms of gene expression-mediated invasion success using C. savignyi as a model species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 576, Issue 1, Part 1, 15 January 2016, Pages 79–87
نویسندگان
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