کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2817490 1159989 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanistic target of rapamycin in common carp: cDNA cloning, characterization, and tissue expression
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Mechanistic target of rapamycin in common carp: cDNA cloning, characterization, and tissue expression
چکیده انگلیسی

The mechanistic target of rapamycin (mTOR) plays a key role in growth and development. In the present study, a cDNA encoding mTOR protein was identified from common carp Cyprinus carpio muscle. The open reading frame of this cDNA encodes 2515 deduced amino acid residues that showed high sequence similarity with its zebrafish Danio rerio counterparts. Bioinformatic analysis showed that the protein belongs to the PI-3 kinase family. The putative protein has FAT, FRB, PI3Kc, and FATC domains, which are highly conserved among the vertebrate orthologs. Real-time quantitative PCR analysis revealed that the abundance of mTOR mRNA was the highest in the heart at 18–31 g and muscle at 31–75 g of common carp. As the common carp grew (18–40 g), the mTOR expression gradually decreased in the hepatopancreas and heart, but increased in the muscle, hind kidney, spleen, gill, head kidney, foregut, midgut, and hindgut. Then the mTOR expression kept a constant in all examined tissues during the common carp grew (40–75 g). The present study identified the mTOR gene and determined its gene expression profile in various tissues of the common carp with body weight increased. These findings will help better understand the biological role of mTOR in the juvenile fish.


► We cloned the ORF cDNA of mTOR gene from common carp muscle for the first time.
► Sequence analysis indicated that common carp mTOR belonged to the PIKK family.
► Common carp mTOR mRNA was detected remarkably high in the muscle and heart.
► Measured changes of mTOR expression in various tissues with weight increasing

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 512, Issue 2, 10 January 2013, Pages 566–572
نویسندگان
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