کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2822995 | 1570172 | 2015 | 8 صفحه PDF | دانلود رایگان |

• It is the first report of genetic analysis of O. tsutsugamushi over South East Asia.
• O. tsutsugamushi is present as a single metapopulation.
• Ancestral alleles are maintained in the population.
• Mites might play a significant role in the genetic structure.
• Efforts should be increased towards early diagnostic for fast treatment.
Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause of febrile illness in rural area of Asia–Pacific region. A multi-locus sequence typing (MLST) analysis was performed on strains isolated from human patients from 3 countries in Southeast Asia: Cambodia, Vietnam and Thailand. The phylogeny of the 56-kDa protein encoding gene was analyzed on the same strains and showed a structured topology with genetically distinct clusters. MLST analysis did not lead to the same conclusion. DNA polymorphism and phylogeny of individual gene loci indicated a significant level of recombination and genetic diversity whereas the ST distribution indicated the presence of isolated patches. No correlation was found with the geographic origin. This work suggests that weak divergence in core genome and ancestral haplotypes are maintained by permanent recombination in mites while the 56-kDa protein gene is diverging in higher speed due to selection by the mammalian immune system.
Journal: Infection, Genetics and Evolution - Volume 31, April 2015, Pages 1–8