Mannose-binding lectin gene polymorphism and risk factors for cardiovascular disease in postmenopausal women

• Evaluation the association of genetic polymorphism of MBL2 as a risk factor for cardiovascular disease (CVD) in 311 postmenopausal women.
• The codon 54 polymorphic allele was found in 25.8% women is associated with the presence of hypertension and insulin resistance.
• This is a possible mechanism of a decreased ability to activate the complement cascade for subsequent production of MBL binding to pro-inflammatory mediators, thereby decreasing inflammation and reducing the risk of vascular damage and/or insulin resistance.

BackgroundInflammatory responses may be altered in postmenopausal women and predispose to cardiovascular disease (CVD). Genetic factors can also influence susceptibility to CVD. Mannose-binding lectin (MBL) is a component of the innate immune system and an activator of the complement cascade. We evaluated the association of genetic polymorphism of MBL (MBL2) on risk factors for CVD in postmenopausal women.MethodsIn this cross-sectional study, 311 Brazilian women (age ≥45 years and amenorrhea ≥12 months) were included. Exclusion criteria: presence of previous or current CVD, insulin dependent diabetes, chronic kidney disease, autoimmune diseases and cancer. Clinical, anthropometric and biochemical assessments were performed to evaluate the cardiovascular risk factors. DNA was extracted from buccal cell and polymorphisms at codons 54 and 57 in the MBL2 were determined by polymerase chain reaction (PCR). For statistical analysis, the chi-square and logistic regression (odds ratio, OR) were used.ResultsThe presence of the polymorphic allele for codon 54 was found in 25.8% of women (A/B = 22.6%, B/B = 3.2%) and for codon 57 in 12.2% (A/C = 10.8%, C/C = 1.4%). The polymorphism at codon 54 was significantly associated with the presence of hypertension (OR 0.55, 95% CI 0.31–0.99, p = 0.044) and insulin resistance assessed by HOMA-IR (OR 0.46, 95% CI 0.24–0.91, p = 0.025). No significant associations were observed between the polymorphism at codon 57 with risk factors for CVD.ConclusionIn postmenopausal women, the polymorphism at codon 54 of the MBL2 was associated with lower risk for hypertension and insulin resistance that are important risk factors for CVD.