کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832783 1163845 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-throughput, functional screening of the anti-HIV-1 humoral response by an enzymatic nanosensor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
High-throughput, functional screening of the anti-HIV-1 humoral response by an enzymatic nanosensor
چکیده انگلیسی

The impact of antibodies on the target's epitope conformation is a major determinant of HIV-1 neutralization and a potential contributor to disease progression. We explore here a conformation-sensitive enzymatic nanosensor for the high-throughput functional screening of human anti-HIV-1 antibodies in sera. When displaying a model epitope from a gp41 immunodominant region (Env residues from 579 to 613), the sensing signal quantitatively distinguishes between adaptive and non-adaptive antibody binding. By using this tool, we have identified IgG4 as the immunoglobulin subpopulation most efficient in the structural modification of the target epitope.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 43, Issue 13, May 2006, Pages 2119–2123
نویسندگان
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