کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2833656 | 1570797 | 2016 | 15 صفحه PDF | دانلود رایگان |
• Molecular phylogenetic analyses and GISH results revealed allopolyploid origin of C. quinoa and C. berlandieri.
• C. quinoa and C. berlandieri might have shared very similar parental species.
• Directional and uniparental loss of rDNA loci was revealed for both C. berlandieri and C. quinoa.
• C. quinoa and C. berlandieri genome sizes were additive when compared with putative parent species.
Most of the cultivated chenopods are polyploids, but their origin and evolutionary history are still poorly understood. Phylogenetic analyses of DNA sequences of four plastid regions, nrITS and nuclear 5S rDNA spacer region (NTS) of two tetraploid chenopods (2n = 4x = 36), Andean C. quinoa and North American C. berlandieri, and their diploid relatives allowed inferences of their origin. The phylogenetic analyses confirmed allotetraploid origin of both tetraploids involving diploids of two different genomic groups (genomes A and B) and suggested that these two might share very similar parentage.The hypotheses on the origin of the two allopolyploid species were further tested using genomic in situ hybridization (GISH). Several diploid Chenopodium species belonging to the two lineages, genome A and B, suggested by phylogenetic analyses, were tested as putative parental taxa. GISH differentiated two sets of parental chromosomes in both tetraploids and further corroborated their allotetraploid origin. Putative diploid parental taxa have been suggested by GISH for C. quinoa and C. berlandieri. Genome sizes of the analyzed allotetraploids fit nearly perfectly the expected additive values of the putative parental taxa. Directional and uniparental loss of rDNA loci of the maternal A-subgenome was revealed for both C. berlandieri and C. quinoa.
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Journal: Molecular Phylogenetics and Evolution - Volume 100, July 2016, Pages 109–123