کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2864481 1573199 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Attenuating Ischemia-Induced H9c2 Myoblasts Apoptosis by Therapeutic Hypothermia
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Attenuating Ischemia-Induced H9c2 Myoblasts Apoptosis by Therapeutic Hypothermia
چکیده انگلیسی
Although inducing mild hypothermia (32 °C) in animal models of cardiac arrest with highly attenuated cardiac and neurological injury, the protective effects of hypothermia molecular mechanisms were not fully elucidated, and thus, were examined here on the H9c2 rat ventricular myoblasts that underwent cell loss as well as apoptosis in conditions of simulated ischemia, represented by serum withdrawal plus hypoxia. The H9c2 cells apoptosis was evidenced by flow cytometry-, DNA fragmentation-, and caspase 3 activation-increased apoptotic cells (Annexin-V positive and propidium iodide negative). For the simulated ischemia, both cell loss and apoptosis of these cardiomyoblasts were associated with downregulated small molecular weight proteins (heat shock protein 20, heat shock protein 27, and αB-crystallin). Mild hypothermia significantly reduced the ischemia-induced apoptosis, small molecular weight proteins downregulation, and cell viability cut. Conclusively, hypothermia may inhibit simulated ischemia-induced apoptosis in cardiomyocytes by restoring normal small molecular weight proteins expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The American Journal of the Medical Sciences - Volume 339, Issue 3, March 2010, Pages 258-265
نویسندگان
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