کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2866175 1573482 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Asbestos-Induced Peribronchiolar Cell Proliferation and Cytokine Production Are Attenuated in Lungs of Protein Kinase C-δ Knockout Mice
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Asbestos-Induced Peribronchiolar Cell Proliferation and Cytokine Production Are Attenuated in Lungs of Protein Kinase C-δ Knockout Mice
چکیده انگلیسی

The signaling pathways leading to the development of asbestos-associated diseases are poorly understood. Here we used normal and protein kinase C (PKC)-δ knockout (PKCδ−/−) mice to demonstrate multiple roles of PKC-δ in the development of cell proliferation and inflammation after inhalation of chrysotile asbestos. At 3 days, asbestos-induced peribronchiolar cell proliferation in wild-type mice was attenuated in PKCδ−/− mice. Cytokine profiles in bronchoalveolar lavage fluids showed increases in interleukin (IL)-1β, IL-4, IL-6, and IL-13 that were decreased in PKCδ−/− mice. At 9 days, microarray and quantitative reverse transcriptase-polymerase chain reaction analysis of lung tissues revealed increased mRNA levels of the profibrotic cytokine, IL-4, in asbestos-exposed wild-type mice but not PKCδ−/− mice. PKCδ−/− mice also exhibited decreased lung infiltration of polymorphonuclear cells, natural killer cells, and macrophages in bronchoalveolar lavage fluid and lung, as well as increased numbers of B lymphocytes and plasma cells. These changes were accompanied by elevated mRNA levels of immunoglobulin chains. These data show that modulation of PKC-δ has multiple effects on peribronchiolar cell proliferation, proinflammatory and profibrotic cytokine expression, and immune cell profiles in lung. These results also implicate targeted interruption of PKC-δ as a potential therapeutic option in asbestos-induced lung diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The American Journal of Pathology - Volume 170, Issue 1, January 2007, Pages 140–151
نویسندگان
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