Allogeneic Heart Valve Storage Above the Glass Transition at −80°C

These results indicate that VS83-preserved heart valves can be stored and transported at temperatures in the vicinity of −80°C with retention of extracellular matrix integrity and material properties. The VS83 preservation of heart valves at −80°C without the need for liquid nitrogen should result in both decreased manufacturing costs and reduced employee safety hazards. Moreover, it is anticipated that low cell viability may result in less immunogenicity in vivo.