کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2893863 1172422 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The CD163-expressing macrophages recognize and internalize TWEAK: Potential consequences in atherosclerosis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
The CD163-expressing macrophages recognize and internalize TWEAK: Potential consequences in atherosclerosis
چکیده انگلیسی

BackgroundCD163 is a new potential scavenger receptor of Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) which elicits diverse biologic actions involved in atherosclerosis. We have analyzed the importance of TWEAK–CD163 interaction in atherosclerosis.MethodsTWEAK and CD163 interaction was studied in cultured human macrophages. Moreover, TWEAK and CD163 expression was analyzed in carotid atherosclerotic plaques (immunohistochemistry) and plasma (ELISA). We have also assessed their potential association with intima/media thickness (IMT) in asymptomatic subjects.ResultsIn vitro studies revealed that CD163-expressing macrophages can bind and internalize TWEAK protein exogenously added from supernatants. Accordingly, we observed an inverse correlation between the expression of CD163 and TWEAK (r = −0.51; p = 0.008) in the shoulder region of atherosclerotic plaques obtained from 25 patients undergoing carotid endarterectomy. The same trend was observed when we analyzed the plasma concentration of both proteins in 90 subjects free from clinical cardiovascular disease (r = −0.25; p = 0.016) in which carotid ultrasonography was performed to determine IMT. In these subjects, we found a positive correlation between sCD163 and IMT (r = 0.36; p < 0.001) and between sCD163–sTWEAK ratio and IMT (r = 0.51; p < 0.001). This association remained significant after adjusting for traditional cardiovascular risk factors and inflammatory markers explaining 39% (sCD163) or 48% (sCD163–sTWEAK ratio) of IMT variance.ConclusionsOur results suggest that TWEAK–CD163 interaction takes place in vivo, probably decreasing TWEAK plasma concentration. Furthermore, we have observed that CD163–TWEAK plasma ratio is a potential biomarker of clinical and subclinical atherosclerosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Atherosclerosis - Volume 207, Issue 1, November 2009, Pages 103–110
نویسندگان
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