کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2953360 1577463 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phosphorylcholine-Targeting Immunization Reduces Atherosclerosis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Phosphorylcholine-Targeting Immunization Reduces Atherosclerosis
چکیده انگلیسی

ObjectivesThe present study evaluated the effect of phosphorylcholine (PC) immunization on the extent of experimental atherosclerosis.BackgroundImmunization against oxidized lipoprotein (oxLDL) or Streptococcus pneumoconiaereduces atherosclerosis. Phosphorylcholine is the main epitope recognized by both antipneumococcus and anti-oxLDL antibodies. Therefore we reasoned that PC-specific antibodies might play an important role in atherogenesis.MethodsApolipoprotein E knockout mice were immunized with PC every second week over 4 months. At the end of the study, serum antibodies directed to either PC or oxLDL were measured. Splenic and peritoneal B cells were analyzed by flow cytometry. Aortic root atherosclerotic lesions were quantified by morphometry and phenotyped by immunohistochemistry. Immune and control sera were also tested for their effect on foam cell formation in macrophage culture in the presence of oxLDL.ResultsThe PC-immunized mice showed 3-fold increase in titers of anti-PC and -oxLDL antibodies compared with control mice (p < 0.01). The PC-immunized mice also showed a significant increase in the number of splenic mature B cells. The extent of atherosclerotic aorta root lesions was reduced by >40% in the PC-immunized mice (p < 0.01). Immunohistochemistry showed reduced expression of major histocompatibility complex class II antigens (p < 0.05) and the presence of B-cell clusters in plaques of PC-immunized mice. Finally, PC-immune serum was able to reduce macrophage-derived foam cell formation in the presence of oxLDL in vitro.ConclusionsPhosphorylcholine immunization drives a specific humoral immune response that reduces foam cell formation in vitro and is atheroprotective in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American College of Cardiology - Volume 50, Issue 6, 7 August 2007, Pages 540–546
نویسندگان
, , , , , , , , ,