کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2965584 | 1178755 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Habitual short sleep duration and circulating endothelial progenitor cells
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
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چکیده انگلیسی
Chronic short sleep duration has been linked to endothelial dysfunction and increased risk of cardiovascular disease. Circulating endothelial progenitor cells (EPCs) are vital to endogenous vascular repair processes and cardiovascular health. We tested the hypothesis that habituai short sleep duration is associated with impairment in EPC number and function. Cells with phenotypic EPC characteristics were isolated from 37 healthy, sedentary adults: 20 with normal sleep duration (13 M/7 F; age: 59±1 years; sleep duration: 7.7±0.1 h/night) and 17 with short sleep duration (9 M/8 F; 56±2 years; 6.0±0.2 h/night). EPC number was assessedby flow cytometric analysis of the percentage of peripheral blood mononuclear cells negative for CD45 and positive for CD34, VEGFR-2, and CD133 antigens. EPC colony-forming capacity was determinedby colony-formingunit (CFU) assay; migration by Boyden chamber; and intracellular caspase-3 concentrations by immunoassay. There were no significant différences between groups in EPC number (0.001±0.0004 vs. 0.001±0.0003 %), colony-forming capacity (6.1±1.5 vs. 5.4±1.7 CFUs), or migrationto VEGF (1410.1±151.2 vs. 1334.3±111.1 AU). Furthermore, there were no group différences inbasal and staurosporine-stimulated intracellular concentrations of active caspase-3 (0.3±0.03 vs. 0.5±0.1 ng/mL; and 2.9±0.4 vs. 2.7±0.3 ng/mL), a marker of apoptotic susceptibility. Taken together, these data indicate that short sleep duration is not associated with EPC dysfunction in healthy adults. Numerical and functional impairment in circulating EPCs may not contribute to the increased cardiovascular risk with habituai short sleep duration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cardiovascular Disease Research - Volume 2, Issue 2, AprilâJune 2011, Pages 110-114
Journal: Journal of Cardiovascular Disease Research - Volume 2, Issue 2, AprilâJune 2011, Pages 110-114
نویسندگان
Brian R. Weil, Owen J. MacEneaney, Brian L. Stauffer, Christopher A. DeSouza,