Algal cell rupture using high pressure homogenization as a prelude to oil extraction

Research associated with extraction of lipids from algae and the use of algal biomass for transportation fuel production is still in its infancy. Measurement techniques associated with algal systems are still not well established and, as a result, pitfalls exist. This paper discusses several measurement and characterization techniques that were attempted while developing an algal oil extraction system and their effectiveness. Measurements were made primarily to quantify the degree of algal cell breakage using high pressure homogenization – as a prelude to solvent extraction.

► Several measurement techniques to measure algal cell rupture are presented.
► High pressure homogenization proved to be an effective to rupture algal cells.
► Pressure differential and number of passes significantly affected cell breakage.
► Particle sizing was not effective in detecting the degree of cell breakage.
► Counting using a Neubauer improved cell counter proved to be the most effective.