Fibrinolysis alterations in infertile women during controlled ovarian stimulation: Influence of BMI and genetic components

IntroductionOvarian stimulation protocols have been described to induce prothrombotic phenotype through alterations of both coagulation and fibrinolysis pathways. We investigated fibrinolytic changes during ovarian stimulation through a global test (CLT) and PAI-1 and TAFI concentrations at different times of ovarian stimulation procedure, and the influence of polymorphisms in genes encoding for fibrinogen chains (FGA, FGB, FGG), t-PA (PLAT), TAFI (CBP2), FXIII (FXIIA1, FXIIIB), plasminogen (PLG) and PAI-1 (PAI1) on their intermediate phenotype.Materials and methodsWe evaluated fibrinolytic and genetic parameters in 110 infertile women undergoing ovarian stimulation procedure (in vitro fertilization, IVF or intracytoplasmic sperm injection, ICSI). All women were observed during the mid-luteal phase of cycle (T0) and on day 5 (T1), 7 (T2) and 9 (T3) of the ovarian stimulation.ResultsSignificant changes in fibrinolytic parameters from T0 to T3 of ovarian stimulation were found (CLT p = 0.003; TAFI p = 0.009 and PAI-1 p = 0.003). CLT values, TAFI and PAI-1 concentrations significantly increased from baseline to T1 (p < 0.0001, p = 0.01, p = 0.005, respectively), and decreased at T2, but remained higher than those at T0. Moreover, at baseline overweight women showed longer CLT, higher TAFI and PAI-1 concentrations than normal weight women, as well as at T1 two-fold longer CLT and higher PAI-1 concentrations were observed (p = 0.001 and p = 0.05, respectively). Significant differences of TAFI and PAI-1 concentrations during ovarian stimulation according to TAFI and PAI1 polymorphisms were observed.ConclusionsThis study shows alterations of fibrinolysis and suggests the contribution of TAFI and PAI1 genes in modulating fibrinolysis changes during the ovarian stimulation cycle.