کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3035866 1579583 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
NAD(P)H oxidase-induced oxidative stress in sympathetic ganglia of apolipoprotein E deficient mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
NAD(P)H oxidase-induced oxidative stress in sympathetic ganglia of apolipoprotein E deficient mice
چکیده انگلیسی
Superoxide anion (O2
- −) is increased throughout the arterial wall in atherosclerosis. The oxidative stress contributes to lesion formation and vascular dysfunction. In the present study, we tested the hypothesis that NAD(P)H oxidase-derived O2
- − is increased in nodose sensory ganglia and sympathetic ganglia of apolipoprotein E deficient (apoE−/−) mice, an established animal model of atherosclerosis. O2
- − measured ex vivo by L-012-enhanced chemiluminescence was increased by 79 ± 17% in whole sympathetic ganglia from apoE−/− mice (n = 5) compared with sympathetic ganglia from control mice (n = 5) (P < 0.05). In contrast, O2
- − was not elevated in nodose ganglia from apoE−/− mice. Dihydroethidium staining confirmed the selective increase in O2
- − in sympathetic ganglia of apoE−/− mice, and revealed the contribution of both neurons and non-neuronal cells to the O2
- − generation. We investigated the enzymatic source of increased O2
- − in sympathetic ganglia of apoE−/− mice. The mRNA expression of gp91phox, p22phox, p67phox, and p47phox subunits of NAD(P)H oxidase measured by real time RT-PCR was increased ∼ 3-4 fold in sympathetic ganglia of apoE−/− mice (n = 5) compared with control ganglia (n = 5). NADPH oxidase activity measured by lucigenin chemiluminescence was increased by 68 ± 12% in homogenates of sympathetic ganglia from apoE−/− mice (n = 7) compared with control ganglia (n = 7) (P < 0.05). The results identify sympathetic ganglia as a novel site of oxidative stress in atherosclerosis, and suggest that upregulation of NAD(P)H oxidase is the source of increased O2
- − generation. We speculate that oxidative stress in sympathetic ganglia may contribute to impaired baroreflex control of sympathetic nerve activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Autonomic Neuroscience - Volumes 126–127, 30 June 2006, Pages 285-291
نویسندگان
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