Lipopolysaccharide preconditioning prevents acceleration of kindling epileptogenesis induced by traumatic brain injury

• 10–20% of symptomatic epilepsies are post-traumatic (PTE).
• No therapy for prevention of PTE is yet available.
• We found that traumatic brain injury (CCI) accelerates kindling epileptogenesis in rats.
• Microglia preconditioning by LPS prevents the acceleration of kindling rate.
• LPS prevents hippocampal IL-1β and TNF-α over-expression and neuronal death.

10–20% of symptomatic epilepsies are post-traumatic. We examined effect of LPS preconditioning on epileptogenesis after controlled cortical impact (CCI). LPS (0.01, 0.1 and 0.5 mg/kg) was injected i.p. to rats 5 days before induction of CCI to parieto-temporal cortex. Kindling started 24 h after CCI by i.p. injection of 30 mg/kg of pentylenetetrazole every other day until manifestation of 3 consecutive generalized seizures. CCI injury accelerated the rate of kindled seizures acquisition. LPS (0.1 and 0.5 mg/kg) prevented the acceleration of kindling. LPS preconditioning significantly decreased IL-1β and TNF-α over-expression and the number of damaged neurons in the hippocampus of traumatic rats.

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