کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3065755 1580486 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of IL-10 expression by upstream stimulating factor (USF-1) in glioma-associated microglia
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Regulation of IL-10 expression by upstream stimulating factor (USF-1) in glioma-associated microglia
چکیده انگلیسی

Understanding the local CNS immune response to neoplasms is essential in the development of immune-based treatments for malignant brain tumors. Using rodent glioma models, we have recently found tumor-associated microglia/macrophages (MG/MP) to be less responsive to known MG/MP activators such as CpG, LPS and IFN-γ. To understand the mechanism of MG/MP suppression, nuclear extracts from rodent intracranial C6 gliomas, C6 glioma-associated MG/MP, normal brain, and normal MG/MP were obtained and studied using Electrophoretic Mobility Shift Assay (EMSA). Among the nuclear factors studied (AP-1, IRF, USF-1 and Stat-1) only USF-1, which is constitutively expressed in most cells, was down-regulated in tumor-associated MG/MP, but not normal MG/MP. Because tumor-associated MG/MP had higher expression of IL-10 (but not TNF-α or TGF-β), we evaluated the role of USF-1 on IL-10 expression. siRNA mediated inhibition of USF-1 expression in primary MG/MP cultures resulted in up-regulation of IL-10 mRNA but not TNF-α or TGF-β. These findings suggest that USF-1 may play a role in IL-10 regulation in MG/MP in brain tumors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 184, Issues 1–2, March 2007, Pages 188–197
نویسندگان
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