کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
313 25 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction and quantification of collagen fiber alignment in a three-dimensional hydroxyapatite–collagen composite scaffold
ترجمه فارسی عنوان
القاء و اندازه گیری فیبر کلاژن در تراز هیدروکسی آپاتیتا کلاژن کامپوزیت
کلمات کلیدی
کامپوزیت کلاژن هیدروکسی آپاتیت، تراز کلاژن، تقسیم کلاژن کوانتومی، روش تبدیل فوریه، میکروسکوپ نور پلاریزه
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

Hydroxyapatite–collagen composite scaffolds are designed to serve as a regenerative load bearing replacement that mimics bone. However, the material properties of these scaffolds are at least an order of magnitude less than that of bone and subject to fail under physiological loading conditions. These scaffolds compositionally resemble bone but they do not possess important structural attributes such as an ordered arrangement of collagen fibers, which is a correlate to the mechanical properties in bone. Furthermore, it is unclear how much ordering of structure is satisfactory to mimic bone. Therefore, quantitative methods are needed to characterize collagen fiber alignment in these scaffolds for better correlation between the scaffold structure and the mechanical properties. A combination of extrusion and compaction was used to induce collagen fiber alignment in composite scaffolds. Collagen fiber alignment, due to extrusion and compaction, was quantified from polarized light microscopy images with a Fourier transform image processing algorithm. The Fourier transform method was capable of resolving the degree of collagen alignment from polarized light images. Anisotropy indices of the image planes ranged from 0.08 to 0.45. Increases in the degree of fiber alignment induced solely by extrusion (0.08–0.25) or compaction (0.25–0.44) were not as great as those by the combination of extrusion and compaction (0.35–0.45). Additional measures of randomness and fiber direction corroborate these anisotropy findings. This increased degree of collagen fiber alignment was induced in a preferred direction that is consistent with the extrusion direction and parallel with the compacted plane.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 17, 15 April 2015, Pages 26–35
نویسندگان
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