کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
31332 44742 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ECHO probes: Fluorescence emission control for nucleic acid imaging
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
ECHO probes: Fluorescence emission control for nucleic acid imaging
چکیده انگلیسی

The understanding of the regulation of the mechanisms via which genomic information dictates cellular behaviors has become a great challenge of the postgenomic era. Tools that allow sensitive, quantitative, and real-time detection of specific transcripts, as well as the study of spatiotemporal gene regulation in living cells, are being developed. In this article, we review recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies. In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D514) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. Multicolor detection and auxiliary functional modules have been built into ECHO probes to accommodate a broad range of biological applications.


► Excitonic interaction suppresses fluorescence emission of thiazole orange homodimers.
► Suppression release in recognition of target nucleic acids robustly turns on multicolor fluorescence.
► Versatile functional modules allow a broad range of applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology C: Photochemistry Reviews - Volume 13, Issue 2, June 2012, Pages 112–123
نویسندگان
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