Harnessing recombination to speed adaptive evolution in Escherichia coli

Evolutionary engineering typically involves asexual propagation of a strain to improve a desired phenotype. However, asexual populations suffer from extensive clonal interference, a phenomenon where distinct lineages of beneficial clones compete and are often lost from the population given sufficient time. Improved adaptive mutants can likely be generated by genetic exchange between lineages, thereby reducing clonal interference. We present a system that allows continuous in situ recombination by using an Esherichia coli F-based conjugation system lacking surface exclusion. Evolution experiments revealed that Hfr-mediated recombination significantly speeds adaptation in certain circumstances. These results show that our system is stable, effective, and suitable for use in evolutionary engineering applications.

► E. coli system that facilitates interclonal genetic exchange in situ during adaptive evolution.
► Expedite the process of adaptive evolution for complex phenotypes.
► Broadly applicable for evolutionary engineering for strain development.