کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3167312 | 1199361 | 2010 | 10 صفحه PDF | دانلود رایگان |
ObjectiveThe aim of this study was to investigate the effects of nano- or submicro topography and fluoride ion on the biology of osteoblasts.Study designPure Ti plates were sandblasted, etched with an HCl/H2SO4 solution (control surface) and then etched in a diluted HF/HNO3 solution (test surface). MC3T3-E1 cells attached, spread, and proliferated on both surfaces.ResultsThe alkaline phosphatase activity was evidently higher for the test surface than for control surface after 4 and 7 days of cell culture. Real-time PCR showed significant increases in type I collagen and osteocalcin gene expression in osteoblast growth on the test surface after 4 days of culture compared with the control surface.ConclusionWith nanotopography and fluoride, hydrogen ions might improve MC3T3-E1 cell proliferation and differentiation during the early stages of cell culture.
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 110, Issue 4, October 2010, Pages e13–e22