Cytotoxicity evaluation of endosequence root repair material

ObjectiveThe purpose of this study was to evaluate the cytotoxicity of EndoSequence Root Repair Material (Brasseler USA, Savannah, GA) and compare it with gray and white MTA.Study designSamples of 2 mg freshly mixed or set gray MTA (GMTA), white MTA (WMTA), EndoSequence Root Repair Material (ERRM), and AH26 were eluted with 300, 600, and 1,000 μL cell culture medium for 24 and 72 hours. L929 cells were seeded into 96-well plates at 3 × 104 cells/well and incubated with 100 μL elute from each elute group. Cells cultured only with culture medium served as negative control. AH26 was used as positive control. After 24 hours' incubation, cell cytotoxicity was evaluated by MTT assay. Cell viability was calculated as percentage of the control group. The results were analyzed with 1-way analysis of variance.ResultsFor both set and fresh samples, there were no significant cell viability differences among GMTA, WMTA, and ERRM. Cell viability in the AH26 group was less than in all of the other 3 materials.ConclusionThis study suggests that ERRM may have cell viability similar to GMTA and WMTA in both set and fresh conditions.