Early gene expression analyzed by a genome microarray and real-time PCR in osteoblasts cultured with a 4-META/MMA-TBB adhesive resin sealer

ObjectivesAdhesive resin sealer systems have been applied in endodontics to seal the root canal system. This study was designed to confirm the mechanism of intracellular molecular events in an in vitro cell culture system with a 4-methacryloxyethyl trimellitate anhydride/methylmethacrylate–tri-n-butyl borane (4-META/MMA-TBB) adhesive resin sealer.Study designThe gene expression patterns relating to cell growth and differentiation were examined using a human genome expression microarray and real-time polymerase chain reaction analyses in hard tissue–forming osteoblasts cultured with and without a 4-META/MMA-TBB resin sealer.ResultsThere was no significant difference in the cell number between the control and adhesive sealer groups. An increased expression of integrin β, transforming growth factor β–related protein, craniofacial development protein 1, and PI3K genes was demonstrated. The integrin β and PI3K genes showed extremely high ratios.ConclusionsThe signal transduction pathway, at least through the PI3K/Akt cascade for cell proliferation and differentiation, can be controlled by some components of this type of adhesive resin sealer.