The cytotoxicity of self-etching primer bonding agents in vitro

ObjectiveThis study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond.Study designThe test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp–derived cells transfected with Simian virus 40 Large T antigen and perfusion condition were conducted in this experiment. Cell viability after exposure to the bonding agents was determined by dimethylthiazolediphenyltetrazolium bromide (MTT) assay.ResultsThe results revealed that cell survival with the above-mentioned bonding agents was 99.66%, 72.59%, and 10.65%, respectively. i Bond is the most toxic material (P < .05). Xeno III is less toxic than i Bond but more toxic than Optibond Solo Plus SE primer (P < .05).ConclusionsAmong the 3 test self-etching bonding systems, Optibond Solo Plus SE primer should be selected in cases where the remaining dentin above pulp tissue is 0.5 mm or less.