کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3168912 | 1199438 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide–induced interleukin-8 expression in human pulp cells Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide–induced interleukin-8 expression in human pulp cells](/preview/png/3168912.png)
ObjectiveThis study examined the effect of nitric oxide (NO) on interleukin-8 (IL-8) production and the involvement of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways in primary cultured human pulp cells.Study designIL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IκB degradation and phosphorylation were determined by western blotting.ResultsSodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IκB, and activation of NF-κB. Furthermore, inhibition of the ERK, p38, and NF-κB pathways blocked SNP-induced IL-8 secretion.ConclusionHuman pulp cells showed NO-induced IL-8 expression via the MAPK and NF-κB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions.
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 105, Issue 5, May 2008, Pages 654–660